Journal article

Publication year: 1988

Pages: 107-111

Journal: Plant Cell, Tissue and Organ Culture

Volume number: 15

Issue number: 2

ISSN: 0167-6857

eISSN: 1573-5044

DOI Link: https://doi.org/10.1007/BF00035752

Publisher: Springer

Abstract: 
Cotyledon explants of Brassica tournefortii L. were excised from germinated seedlings and cultured on Murashige and Skoog''s [6] basal medium supplemented with various combinations of cytokinins and auxins. Both cytokinin and auxin were required for induction of shoot organogenesis. Of the three cytokinins tested (in combination with a low concentration of IAA), kinetin [KIN] was found to be the best for shoot regeneration. On this medium, cotyledonary explants invariably underwent callusing followed by multiple shoot formation. NAA in combination with any of the three cytokinins yielded a reduced number of shoots or none, but favoured good callus growth. Callus so produced also regenerated shoots when subcultured on media containing high concentration of KIN or ZEA [zeatin] and low concentration of IAA. Shoots were rooted during prolonged incubation on the same medium or on MS medium free of growth regulators. Mature plants were grown in the greenhouse.

Harvard Citation style: JAIN, R., CHOWDHURY, J. and FRIEDT, W. (1988) ORGANOGENESIS AND PLANT FORMATION FROM COTYLEDON EXPLANT CULTURES OF WILD TURNIP RAPE (BRASSICA-TOURNEFORTII L), Plant Cell, Tissue and Organ Culture, 15(2), pp. 107-111. https://doi.org/10.1007/BF00035752

APA Citation style: JAIN, R., CHOWDHURY, J., & FRIEDT, W. (1988). ORGANOGENESIS AND PLANT FORMATION FROM COTYLEDON EXPLANT CULTURES OF WILD TURNIP RAPE (BRASSICA-TOURNEFORTII L). Plant Cell, Tissue and Organ Culture. 15(2), 107-111. https://doi.org/10.1007/BF00035752