Journal article

Publication year: 1987

Pages: 433-437

Journal: Planta

Volume number: 171

Issue number: 4

ISSN: 0032-0935

eISSN: 1432-2048

DOI Link: https://doi.org/10.1007/BF00392289

Publisher: Springer

Abstract: 
The calcium vesicles of the green alga Mougeotia (G. Wagner and R. Rossbacher, 1980, Planta 149, 298-305) were isolated from characterization in vitro by fractionation of algal homogenate on sucrose density gradients. A new technique, based on vital staining by neutral red or rhodamine B, permitted isolation. Minimum dye binding to the calcium vesicles prevented disintegration, and for isolation a single, thoroughly defined centrifugation step sufficed, facilitated by the exceptionally high vesicular density of .gtoreq. 1.3 g .cntdot. cm-3. Neutral red in particular seems to be accumulated by the vesicles via hydrogen bonds to abundant phenolic hydroxyl groups which, reversibly bound to an as yet undefined vesicle core, may well provide coordination sites for the observed calcium binding.

Harvard Citation style: GROLIG, F. and WAGNER, G. (1987) VITAL STAINING PERMITS ISOLATION OF CALCIUM VESICLES FROM THE GREEN-ALGA MOUGEOTIA, Planta, 171(4), pp. 433-437. https://doi.org/10.1007/BF00392289

APA Citation style: GROLIG, F., & WAGNER, G. (1987). VITAL STAINING PERMITS ISOLATION OF CALCIUM VESICLES FROM THE GREEN-ALGA MOUGEOTIA. Planta. 171(4), 433-437. https://doi.org/10.1007/BF00392289