Journal article

Publication year: 1987

Pages: 430-436

Journal: Journal of Cancer Research and Clinical Oncology

Volume number: 113

Issue number: 5

ISSN: 0171-5216

eISSN: 1432-1335

DOI Link: https://doi.org/10.1007/BF00390036

Publisher: Springer

Abstract: 
Preneoplastic liver lesions were produced in female Wistar rats by oral administration of 2-acetyl-aminofluorene for 165 days succeeded by a carcinogen-free standard diet up to 420 days. During the treatment numerous altered hepatic foci (AHF) and hyperplastic nodules (HN) were detected histochemically be a focal decrease or lack of adenosine-5-triphosphatase and glucose-6-phosphatase (G-6-Pase) activities. In addition, the immunohistochemically demonstrable amount of L-type pyruvate kinase was clearly reduced. The histochemically demonstrated decrease of G-6-Pase was substantiated by microbiochemical determination of the enzyme activity in microdissected material. Moreover, during the experimental period a continuous decrease in glucokinase and an increase in hexokinase was detected microbiochemically within AHF and HN. These alterations indicate a shift in the carbohydrate metabolism from gluconeogenesis to glucose utilization and pentose-phosphate-pathway for biosynthesis of nucleic acids. Beside other oncofetal markers, HK may be used as indicator of the early stages of liver carcinogenesis.

Harvard Citation style: FISCHER, G., RUSCHENBURG, I., EIGENBRODT, E. and KATZ, N. (1987) DECREASE IN GLUCOKINASE AND GLUCOSE-6-PHOSPHATASE AND INCREASE IN HEXOKINASE IN PUTATIVE PRENEOPLASTIC LESIONS OF RAT-LIVER, Journal of Cancer Research and Clinical Oncology, 113(5), pp. 430-436. https://doi.org/10.1007/BF00390036

APA Citation style: FISCHER, G., RUSCHENBURG, I., EIGENBRODT, E., & KATZ, N. (1987). DECREASE IN GLUCOKINASE AND GLUCOSE-6-PHOSPHATASE AND INCREASE IN HEXOKINASE IN PUTATIVE PRENEOPLASTIC LESIONS OF RAT-LIVER. Journal of Cancer Research and Clinical Oncology. 113(5), 430-436. https://doi.org/10.1007/BF00390036