Journal article

Publication year: 1986

Pages: 823-829

Journal: Comparative biochemistry and physiology. Part B: Comparative biochemistry

Volume number: 83

Issue number: 4

ISSN: 1096-4959

eISSN: 1879-1107

DOI Link: https://doi.org/10.1016/0305-0491(86)90154-9

Publisher: Pergamon Press

Abstract: 
Pyruvate kinase isoenzymes type M1 and M2 from dog muscle, lung and tumor have been isolated. The K0.5 for phosphoenol pyruvate have been determined to be 0.04 mM for dog muscle type M1, 0.24 mM for lung type M2 and 0.28 mM for tumor type M2 isoenzymes. The activator constant ka of L-serine is 240 nM from lung and 70 nM from tumor isoenzyme. Consistent with the assumption of a special form of pyruvate kinase M2 in dog tumor cells different isoelectric points and amino acid compositions have been found for the isoenzymes of lung and tumor.

Harvard Citation style: Becker, K., Geyer, H., Eigenbrodt, E. and Schoner, W. (1986) Purification of pyruvate kinase isoenzymes type M1 and M2 from dog (Canis familiaris) and comparison of their properties with those from chicken and rat, Comparative biochemistry and physiology. Part B: Comparative biochemistry, 83(4), pp. 823-829. https://doi.org/10.1016/0305-0491(86)90154-9

APA Citation style: Becker, K., Geyer, H., Eigenbrodt, E., & Schoner, W. (1986). Purification of pyruvate kinase isoenzymes type M1 and M2 from dog (Canis familiaris) and comparison of their properties with those from chicken and rat. Comparative biochemistry and physiology. Part B: Comparative biochemistry. 83(4), 823-829. https://doi.org/10.1016/0305-0491(86)90154-9