Journal article

Publication year: 1984

Pages: 62-67

Journal: Planta

Volume number: 162

Issue number: 1

ISSN: 0032-0935

eISSN: 1432-2048

DOI Link: https://doi.org/10.1007/BF00397422

Publisher: Springer

Abstract: 
A soluble protein was isolated from Mougeotia by chlorpromazine-sepharose 4 B affinity chromatography. The protein matches the properties of calmodulin in terms of heat stability, Ca2+-dependent electrophoretic mobility in sodium-dodecyl-sulfate polyacrylamide gels and its ability to activate cyclic nucleotide phosphodiesterase in a Ca2+-dependent manner. Phytochrome-mediated chloroplast reorientational movement in Mougeotia was inhibited by the calmodulin antagonist trifluoperazine, a hydrophobic compound, or N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a hydrophilic compound; 50% inhibition (IC50) of chloroplast movement is caused by 20-50 .mu.mol l-1 trifluoperazine or 100 .mu.mol l-1 W-7. The Ca2+-calmodulin may act as an intermediate in the chloroplast reorientational response in Mougeotia governed by phytochrome.

Harvard Citation style: WAGNER, G., VALENTIN, P., DIETER, P. and MARME, D. (1984) IDENTIFICATION OF CALMODULIN IN THE GREEN-ALGA MOUGEOTIA AND ITS POSSIBLE FUNCTION IN CHLOROPLAST REORIENTATIONAL MOVEMENT, Planta, 162(1), pp. 62-67. https://doi.org/10.1007/BF00397422

APA Citation style: WAGNER, G., VALENTIN, P., DIETER, P., & MARME, D. (1984). IDENTIFICATION OF CALMODULIN IN THE GREEN-ALGA MOUGEOTIA AND ITS POSSIBLE FUNCTION IN CHLOROPLAST REORIENTATIONAL MOVEMENT. Planta. 162(1), 62-67. https://doi.org/10.1007/BF00397422