Journal article

Publication year: 1984

Pages: 127-142

Journal: Cytologia

Volume number: 49

Issue number: 1

ISSN: 0011-4545

Open access status: Bronze

DOI Link: https://doi.org/10.1508/cytologia.49.127

Publisher: Japan Mendel Society

Abstract: 
Formation of the egg-membrane and the attaching-filaments of the marine goby P. minutus was studied with EM autoradiography using the tracer L-[3H]-proline. The egg-membrane is formed in the chronological sequence as follows: 1st labeled material is found in the oocytoplasm by 15 min post-injection, in Golgi bodies by 30-60 min, in dense-cored vesicles and pinocytotic vesicles by 3 h and the substance of the egg-membrane by 12-24 h post-injection. The material forming the egg-membrane is probably synthesized in the oocytoplasm, concentrated in the Golgi bodies and then transfered via dense-cored and pinocytotic vesicles to the developing egg-membrane. In the follicle epithelium 1st label appears in stage II and III oocytes at 30 min post-injection, and reaches the maximum concentration in the endoplasmic reticulum by 24 h. The egg-membrane of P. minutus is produced by the oocyte itself, whereas the attaching-filaments are synthesized by the endoplasmic reticulum of the follicle cells. Therefore, according to the classical definition of Waldeyer (1906), the egg-membrane of this species is a primary envelope, while the attaching-filaments represent a secondary envelope or chorion.

Harvard Citation style: RIEHL, R. (1984) THE OOCYTES OF THE GOBY POMATOSCHISTUS-MINUTUS .4. ELECTRON-MICROSCOPIC AUTORADIOGRAPHY OF [H-3] PROLINE INCORPORATION, Cytologia, 49(1), pp. 127-142. https://doi.org/10.1508/cytologia.49.127

APA Citation style: RIEHL, R. (1984). THE OOCYTES OF THE GOBY POMATOSCHISTUS-MINUTUS .4. ELECTRON-MICROSCOPIC AUTORADIOGRAPHY OF [H-3] PROLINE INCORPORATION. Cytologia. 49(1), 127-142. https://doi.org/10.1508/cytologia.49.127