Journalartikel

Jahr der Veröffentlichung: 1984

Seiten: 242-246

Zeitschrift: Gynecologic and Obstetric Investigation

Bandnummer: 17

Heftnummer: 5

ISSN: 0378-7346

eISSN: 1423-002X

DOI Link: https://doi.org/10.1159/000299156

Verlag: Karger Publishers

Abstract: 
In particular membrane fractions of human rabbit oviductal smooth muscle specific binding sites for the radiolabeled .beta.-adrenergic antagonist [125I]-cyanopindolol (I-CYP) were detectable utilizing the radioligand technique. The binding was saturable, relative to the concentration of protein as well as ligand in the assay. The analysis of the binding kinetics by Scatchard plots revealed a number of .beta.-adrenergic receptor sites of 40 fmol I-CYP bound per mg protein with a KD of 20 pM. In competition binding studies with .beta.2-adrenergic agonists and antagonists [phentolamine, phenylephrine, isoproterenol and propranolol, HI-42BS, 32-468 HMO] the receptors in their pharmacological range corresponded to the .beta.2-subtype of .beta.-adrenergic receptors. Within the oviduct the ampulla possessed 70% of all traceable .beta.-adrenergic receptor sites.

Harvard-Zitierstil: KLEINSTEIN, J. (1984) BINDING-PROPERTIES OF BETA-ADRENERGIC RECEPTORS IN OVIDUCTAL SMOOTH-MUSCLE MEMBRANES, Gynecologic and Obstetric Investigation, 17(5), pp. 242-246. https://doi.org/10.1159/000299156

APA-Zitierstil: KLEINSTEIN, J. (1984). BINDING-PROPERTIES OF BETA-ADRENERGIC RECEPTORS IN OVIDUCTAL SMOOTH-MUSCLE MEMBRANES. Gynecologic and Obstetric Investigation. 17(5), 242-246. https://doi.org/10.1159/000299156