Journal article

AN IMPROVED METHOD FOR THE DETERMINATION OF THE NUMBER OF ENDOSPERM CELLS IN WHEAT GRAINS


Authors listFEUCHT, D; HOFNER, W

Publication year1983

Pages697-704

JournalJournal of Plant Nutrition and Soil Science

Volume number146

Issue number6

ISSN0044-3263

DOI Linkhttps://doi.org/10.1002/jpln.19831460604

PublisherWiley-VCH Verlag


Abstract
A method is described which allows a clear and quantitative determination of endosperm cells in wheat grains even during the late stages of grain filling. After treatment with pectinase the tissue of the endosperm disintegrates in single cells, in the same step, the starch in the endosperm cells is dissolved. The nuclei of the endosperm cells are stained with hematoxylin, drawn by vacuum on to a millipore filter, and counted in the still recognizable cell structures under a microscope. This advantageous for exact counting of all the endosperm cells.



Citation Styles

Harvard Citation styleFEUCHT, D. and HOFNER, W. (1983) AN IMPROVED METHOD FOR THE DETERMINATION OF THE NUMBER OF ENDOSPERM CELLS IN WHEAT GRAINS, Journal of Plant Nutrition and Soil Science = Zeitschrift für Pflanzenernährung und Bodenkunde, 146(6), pp. 697-704. https://doi.org/10.1002/jpln.19831460604

APA Citation styleFEUCHT, D., & HOFNER, W. (1983). AN IMPROVED METHOD FOR THE DETERMINATION OF THE NUMBER OF ENDOSPERM CELLS IN WHEAT GRAINS. Journal of Plant Nutrition and Soil Science = Zeitschrift für Pflanzenernährung und Bodenkunde. 146(6), 697-704. https://doi.org/10.1002/jpln.19831460604


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