Journal article
Authors list: FEUCHT, D; HOFNER, W
Publication year: 1983
Pages: 697-704
Journal: Journal of Plant Nutrition and Soil Science
Volume number: 146
Issue number: 6
ISSN: 0044-3263
DOI Link: https://doi.org/10.1002/jpln.19831460604
Publisher: Wiley-VCH Verlag
Abstract:
A method is described which allows a clear and quantitative determination of endosperm cells in wheat grains even during the late stages of grain filling. After treatment with pectinase the tissue of the endosperm disintegrates in single cells, in the same step, the starch in the endosperm cells is dissolved. The nuclei of the endosperm cells are stained with hematoxylin, drawn by vacuum on to a millipore filter, and counted in the still recognizable cell structures under a microscope. This advantageous for exact counting of all the endosperm cells.
Citation Styles
Harvard Citation style: FEUCHT, D. and HOFNER, W. (1983) AN IMPROVED METHOD FOR THE DETERMINATION OF THE NUMBER OF ENDOSPERM CELLS IN WHEAT GRAINS, Journal of Plant Nutrition and Soil Science = Zeitschrift für Pflanzenernährung und Bodenkunde, 146(6), pp. 697-704. https://doi.org/10.1002/jpln.19831460604
APA Citation style: FEUCHT, D., & HOFNER, W. (1983). AN IMPROVED METHOD FOR THE DETERMINATION OF THE NUMBER OF ENDOSPERM CELLS IN WHEAT GRAINS. Journal of Plant Nutrition and Soil Science = Zeitschrift für Pflanzenernährung und Bodenkunde. 146(6), 697-704. https://doi.org/10.1002/jpln.19831460604