Journalartikel

Jahr der Veröffentlichung: 1982

Seiten: 486-494

Zeitschrift: Biochimica et Biophysica Acta (BBA)

Bandnummer: 688

Heftnummer: 2

ISSN: 0006-3002

DOI Link: https://doi.org/10.1016/0005-2736(82)90360-1

Verlag: Elsevier

Abstract: 
Palytoxin caused within min a temperature-dependent K+ loss from human and rat erythrocytes which was followed within h by hemolysis. It decreased the osmotic resistance in a concentration-dependent manner, so that osmotic influences were negligible for K+ release but considerable in hemolysis. External K+ inhibited the Hb release and Rb+ inhibited the release of K+ and Hb. Ca2+ (> 20 .mu.M) and borate (> 5 .mu.M) enhanced the loss of K+ and Hb. With Ca2+ and borate present, the efficacy of palytoxin was raised .apprx. 10,000-fold. Under these conditions, .apprx. 15 palytoxin molecules/human cell triggered a 50% K+ loss over a wide range of cell concentrations. The palytoxin effect was reversible. After depletion from K+ by low concentrations of palytoxin, human cells could be refilled with K+ and resealed. The pores formed by palytoxin were small. They allowed the entrance of Na+ and choline; inositol was largely excluded and Ca2+, sucrose and inulin were excluded. Amphotericin B resembled palytoxin in its ability to cause a considerable prelytic K+ loss and to form small pores. It was .apprx. 1000-fold weaker than palytoxin, was not inhibited by K+ or Rb+, was not activated by Ca2+ or borate and had a negative temperature dependence. Palytoxin represented a novel type of cytolysin.

Harvard-Zitierstil: AHNERTHILGER, G., CHHATWAL, G., HESSLER, H. and HABERMANN, E. (1982) CHANGES IN ERYTHROCYTE PERMEABILITY DUE TO PALYTOXIN AS COMPARED TO AMPHOTERICIN-B, Biochimica et Biophysica Acta (BBA), 688(2), pp. 486-494. https://doi.org/10.1016/0005-2736(82)90360-1

APA-Zitierstil: AHNERTHILGER, G., CHHATWAL, G., HESSLER, H., & HABERMANN, E. (1982). CHANGES IN ERYTHROCYTE PERMEABILITY DUE TO PALYTOXIN AS COMPARED TO AMPHOTERICIN-B. Biochimica et Biophysica Acta (BBA). 688(2), 486-494. https://doi.org/10.1016/0005-2736(82)90360-1