Journalartikel

INVITRO TRANSCRIPTION OF VIROID RNA INTO FULL-LENGTH COPIES BY RNA-DEPENDENT RNA-POLYMERASE FROM HEALTHY TOMATO LEAF TISSUE


AutorenlisteBOEGE, F; ROHDE, W; SANGER, HL

Jahr der Veröffentlichung1982

Seiten185-194

ZeitschriftBioscience Reports

Bandnummer2

Heftnummer3

ISSN0144-8463

eISSN1573-4935

DOI Linkhttps://doi.org/10.1007/BF01116382

VerlagPortland Press


Abstract
RNA-dependent RNA polymerase from healthy tomato plant tissue accepts potato spindle tuber viroid (PSTV) RNA as a template for the in vitro synthesis of full-length RNA copies of the PSTV genome. Viroid transcription requires the presence of Mn2+ and/or Mg2+ ions and is not inhibited by concentrations of 10-5 M .alpha.-amanitin. This is the 1st report of a well-defined product synthesized in vitro by an RNA-dependent RNA polymerase from healthy plants.



Zitierstile

Harvard-ZitierstilBOEGE, F., ROHDE, W. and SANGER, H. (1982) INVITRO TRANSCRIPTION OF VIROID RNA INTO FULL-LENGTH COPIES BY RNA-DEPENDENT RNA-POLYMERASE FROM HEALTHY TOMATO LEAF TISSUE, Bioscience Reports, 2(3), pp. 185-194. https://doi.org/10.1007/BF01116382

APA-ZitierstilBOEGE, F., ROHDE, W., & SANGER, H. (1982). INVITRO TRANSCRIPTION OF VIROID RNA INTO FULL-LENGTH COPIES BY RNA-DEPENDENT RNA-POLYMERASE FROM HEALTHY TOMATO LEAF TISSUE. Bioscience Reports. 2(3), 185-194. https://doi.org/10.1007/BF01116382


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