Journalartikel

Jahr der Veröffentlichung: 1982

Seiten: 181-196

Zeitschrift: Archives of Virology

Bandnummer: 74

Heftnummer: 2-3

ISSN: 0304-8608

eISSN: 1432-8798

DOI Link: https://doi.org/10.1007/BF01314711

Verlag: Springer

Abstract: 
Gel electrophoretic analysis revealed marked alterations in the pattern of acid-extractable proteins from tomato leaves after infection with potato spindle tuber viroid (PSTV), 2 virsues [tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV)] and a fungus (Cladosporium fulvum) when compared to the pattern from healthy leaves. A pathogen-specific appearance of new protein bands was only found after infection with TMV (MW 17,400 and 65,000), CMV (MW 9000 and 8000) and C. fulvum (MW 28,000). With the exception of the TMV coat protein (MW 17,400) it could not be established whether the other 4 proteins are coded for by the corresponding pathogen or by the host. Nine proteins with the apparent MW of 10,000, 11,000, 12,000, 13,000, 14,000, 25,000, 31,000, 33,000 and 38,000 showed an increase in their relative concentration which is most dramatic in the case of the protein with the MW of 14,000 called p14. A decrease was observed in 4 proteins with MW of 14,500, 23,000, 30,000 and 105,000. Since all these alterations could be correlated with the severity of the disease symptoms but not with the nature of the pathogen they must be considered as a general pathophysiological response of the tomato plant to infection and sympton development. A partial purification of the most prominent pathogenesis-related protein p14 from tomato plants is described.

Harvard-Zitierstil: HENRIQUEZ, A. and SANGER, H. (1982) ANALYSIS OF ACID-EXTRACTABLE TOMATO LEAF PROTEINS AFTER INFECTION WITH A VIROID, 2 VIRUSES AND A FUNGUS AND PARTIAL-PURIFICATION OF THE PATHOGENESIS-RELATED PROTEIN-P14, Archives of Virology, 74(2-3), pp. 181-196. https://doi.org/10.1007/BF01314711

APA-Zitierstil: HENRIQUEZ, A., & SANGER, H. (1982). ANALYSIS OF ACID-EXTRACTABLE TOMATO LEAF PROTEINS AFTER INFECTION WITH A VIROID, 2 VIRUSES AND A FUNGUS AND PARTIAL-PURIFICATION OF THE PATHOGENESIS-RELATED PROTEIN-P14. Archives of Virology. 74(2-3), 181-196. https://doi.org/10.1007/BF01314711