Journal article

Publication year: 1981

Pages: 327-336

Journal: Bioscience Reports

Volume number: 1

Issue number: 4

ISSN: 0144-8463

eISSN: 1573-4935

DOI Link: https://doi.org/10.1007/BF01114872

Publisher: Portland Press

Abstract: 
Molecular hybridization by the Northern blot technique in combination with 125I-labeled PSTV [potato spindle tuber viroid] (+) RNA and 32P-labeled PSTV cDNA as probes was applied to detect viroid-specific sequences in healthy and viroid (PSTV)-infected tomato plants. Conditions are described which allow differentiation of (+) and (-) viroid sequences on the basis of the different thermostabilities of the corresponding hybrid molecules. No viroid-specific DNA sequences were detected by this approach. Viroid replication apparently proceeds via complementary RNA intermediates. Of the 7 (-) RNA species found, 6 are apparently larger than the circular viroid (+) RNA and 1 is about the same size as the linear (+) RNA molecule.

Harvard Citation style: ROHDE, W. and SANGER, H. (1981) DETECTION OF COMPLEMENTARY RNA INTERMEDIATES OF VIROID REPLICATION BY NORTHERN BLOT HYBRIDIZATION, Bioscience Reports, 1(4), pp. 327-336. https://doi.org/10.1007/BF01114872

APA Citation style: ROHDE, W., & SANGER, H. (1981). DETECTION OF COMPLEMENTARY RNA INTERMEDIATES OF VIROID REPLICATION BY NORTHERN BLOT HYBRIDIZATION. Bioscience Reports. 1(4), 327-336. https://doi.org/10.1007/BF01114872