Journal article

Publication year: 1980

Pages: 33-43

Journal: Microscopica acta

Volume number: 83

Issue number: 1

ISSN: 0044-376X

Publisher: Hirzel

Abstract: 
The egg-shells of 3 teleosteans (Coregonus nasus, Salmo salar, S. trutta fario) were oxidized in a gas-discharge apparatus with O2 activated at high-frequency. This procedure was followed by analysis with a scanning electron microscope. High-frequency oxidation permits a removal of organic materials layer by layer. Before oxidation, it is advantageous to break the specimens by freeze-fracturing or to cut them with a razor-blade. The resulting relatively smooth surfaces can be etched easier, and clearer pictures may be obtained than with untreated specimens. This is due to the fact that after exposing the surfaces of fish eggs to a known dosage of high-frequency oxidation, they are free of the remains of the follicle epithelium and other unwanted particles. Details are seen, that before were barely visible. Because of the presence of etching effects, the examination of biological specimens at higher magnifications is limited. High-frequency oxidation is a valuable method for the biologists with which, without great expense, one can obtain structure elucidations by scanning electron microscopy.

Harvard Citation style: RIEHL, R., BRUNEGGER, A. and JAKOPIC, E. (1980) APPLICATION OF HIGH-FREQUENCY ACTIVATED OXYGEN IN THE SCANNING ELECTRON-MICROSCOPIC ANALYSIS OF FISH EGGS, MICROSCOPICA ACTA, 83(1), pp. 33-43

APA Citation style: RIEHL, R., BRUNEGGER, A., & JAKOPIC, E. (1980). APPLICATION OF HIGH-FREQUENCY ACTIVATED OXYGEN IN THE SCANNING ELECTRON-MICROSCOPIC ANALYSIS OF FISH EGGS. MICROSCOPICA ACTA. 83(1), 33-43.