Journalartikel

Jahr der Veröffentlichung: 1978

Seiten: 7-12

Zeitschrift: Planta

Bandnummer: 144

Heftnummer: 1

ISSN: 0032-0935

eISSN: 1432-2048

DOI Link: https://doi.org/10.1007/BF00385001

Verlag: Springer

Abstract: 
Using a 14C/3H double-labeling technique, the influence of kinetin on the length of the cell cycle of meristematic cells in haploid and diploid callus cultures of D. innoxia was determined. The total length of the cell cycle of haploid cells as compared to that of diploid cells was reduced by 2.3 h (-kinetin) or 1.4 h (+kinetin). The addition of kinetin to the nutrient solution also reduces cell cycle duration at both ploidy levels. For synchronization of the cell cycle, a fluorodesoxyuridine/thymidine system was successfully employed. Apparently, the reduction of total cell cycle duration of cycling cells due to treatment with kinetin occurred at the expense of the G1 phase. Nevertheless, kinetic seems to exert an influence on the transition of cells from the G2 into the M phase as well.

Harvard-Zitierstil: BLASCHKE, J., FORCHE, E. and NEUMANN, K. (1978) INVESTIGATIONS ON CELL-CYCLE OF HAPLOID AND DIPLOID TISSUE-CULTURES OF DATURA-INNOXIA MILL AND ITS SYNCHRONIZATION, Planta, 144(1), pp. 7-12. https://doi.org/10.1007/BF00385001

APA-Zitierstil: BLASCHKE, J., FORCHE, E., & NEUMANN, K. (1978). INVESTIGATIONS ON CELL-CYCLE OF HAPLOID AND DIPLOID TISSUE-CULTURES OF DATURA-INNOXIA MILL AND ITS SYNCHRONIZATION. Planta. 144(1), 7-12. https://doi.org/10.1007/BF00385001