Journal article

Publication year: 1976

Pages: 403-407

Journal: Physiologia Plantarum

Volume number: 36

Issue number: 4

ISSN: 0031-9317

eISSN: 1399-3054

Publisher: Wiley

Abstract: 
Cell suspension cultures of P. sativum L. derived from root and shoot sections of seedlings were prepared and cultured in defined nutrient medium. Both the cells and the media were analyzed daily for the N-fractions and carbohydrates during the growth period. Specific correlations were found between growth and N and carbohydrate metabolism. At the beginning of the growth cycle, ammonia as compared to nitrate was favored in uptake. An increased uptake of nitrate occurred at the end of the linear growth phase when carbohydrate in the media was depleted. The uptake of sucrose was rapid during the whole growth cycle, only in the range of the linear growth phase the uptake stagnated for 3-4 days. During increased biosynthesis of nitrogenous compounds at the beginning of the growth cycle, up to 7 isoenzymes of glutamate dehydrogenase could be separated by polyacrylamide gel electrophoresis. The isoenzyme pattern changed during the stationary growth phase, especially when the carbohydrate content in the medium decreased. There was some evidence that the isoenzyme pattern was influenced by carbohydrate metabolism.

Harvard Citation style: HARMS, H., PRIESS, I. and PAHLICH, E. (1976) GROWTH, N-METABOLISM AND ISOENZYME PATTERN OF GLUTAMATE-DEHYDROGENASE IN BATCH-CULTURES OF PISUM-SATIVUM CELLS UNDER LIGHT AND DARK CONDITIONS, Physiologia Plantarum, 36(4), pp. 403-407

APA Citation style: HARMS, H., PRIESS, I., & PAHLICH, E. (1976). GROWTH, N-METABOLISM AND ISOENZYME PATTERN OF GLUTAMATE-DEHYDROGENASE IN BATCH-CULTURES OF PISUM-SATIVUM CELLS UNDER LIGHT AND DARK CONDITIONS. Physiologia Plantarum. 36(4), 403-407.