Journal article

Publication year: 1976

Pages: 317-330

Journal: Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Abteilung 1, Reihe B

Volume number: 161

Issue number: 4

ISSN: 0174-3015

Publisher: Fischer

Abstract: 
The long-term effects of inorganic Pb salts on L-A mouse fibroblast cells cultured in vitro are described. Under continuous exposure Pb tolerance developed, even to concentrations exceeding the LD50. The kinetics of the acquirement of resistance are demonstrated. The resistant cells adjusted more quickly to higher doses of the heavy metal than the controls. Low-resistance strains retained the growth characteristics of the parent cells, but with increasing levels of Pb tolerance multiplication rates decreased significantly. After approximately 60 generations in Pb-free environment the cells had to a major degree lost their resistance indicating that the induced Pb tolerance is caused by adaptation rather than by mutation and selection. Pb resistance appears not to depend on Pb exclusion, for the Pb content of resistant cells and Pb-exposed controls was of a comparable order of magnitude.

Harvard Citation style: Fischer, A. (1976) The effect of lead on cells cultivated in vitro. II. Chronic exposure and development of resistance, Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Abteilung 1, Reihe B, 161(4), pp. 317-330

APA Citation style: Fischer, A. (1976). The effect of lead on cells cultivated in vitro. II. Chronic exposure and development of resistance. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Abteilung 1, Reihe B. 161(4), 317-330.