Journalartikel

Jahr der Veröffentlichung: 2023

Zeitschrift: Food chemistry advances

Bandnummer: 2

DOI Link: https://doi.org/10.1016/j.focha.2023.100283

Verlag: Elsevier

Abstract: 

Screening for antibiotic residues in animal-derived food is largely done by in vitro assays. However, a non-negligible number of positive screening results (sum values) remains with no confirmation by high-performance liquid chromatography coupled to high-sophisticated mass spectrometers. To investigate this inconsistency, a hyphenated strategy was sought that combines on the same surface planar chromatography−multi-imaging with antibacterial planar bioassays. Strong antibacterial activity against Gram-positive and Gram-negative bacteria was revealed in four animal-derived food extracts (pig muscle, cow milk, chicken eggs, and honey), which, however, were previously proven to be veterinary drug residue-free. This antibacterial effect was strong, extremely exceeding the effect from a mixture of 81 veterinary drugs spiked at 300 µg/kg, which is about 3 times the maximum residue limit of most of these veterinary drugs. The unknown antibacterial zones were online-eluted to high-resolution mass spectrometry to obtain molecular formulas. Endogenous fatty acids and lipids were identified to be responsible for the strong antibacterial effects, confirmed by co-analyzed reference compounds. These results resolved the methodological inconsistency but also question the prevailing understanding: Current food safety only based on target analysis of regulated antibiotic residues neglects consumer protection when the much stronger antibacterial impact of food on the human microbiome is overlooked.

Harvard-Zitierstil: Mehl, A. and Morlock, G. (2023) Strong antibacterial effects in animal-derived food detected via non-target planar bioassays, Food chemistry advances, 2, Article 100283. https://doi.org/10.1016/j.focha.2023.100283

APA-Zitierstil: Mehl, A., & Morlock, G. (2023). Strong antibacterial effects in animal-derived food detected via non-target planar bioassays. Food chemistry advances. 2, Article 100283. https://doi.org/10.1016/j.focha.2023.100283