Journalartikel

Jahr der Veröffentlichung: 1989

Seiten: 219-229

Zeitschrift: European Journal of Pharmacology

Bandnummer: 168

Heftnummer: 2

ISSN: 0014-2999

eISSN: 1879-0712

DOI Link: https://doi.org/10.1016/0014-2999(89)90568-2

Verlag: Elsevier

Abstract: 
The acetylcholine receptor agonists, acetylcholine (10-5-10-4 M), carbachol (5 .times. 10-6-5 .times. 10-5 M), bethanechol (5 .times. 10-5-5.times.10-4 M) and dimethyphenylpiperazinium (DMPP, 10-5 M) increased the short-circuit current (Isc) in the rat colon descendens by a tetrodotoxin (TTX)-sensitive mechanism. Blockade by TTX was still observed after removal of the submucosa, indicating the involvement of neurons of the mucosal plexus. Hexamethonium (10-5 M) and atropine (10-6 M) were used to distinguish between nicotinic and muscarinic neuronally mediated effects. The inhibitor of choline uptake hemicholinium-3 (1 mM), reversibly inhibited the effect of repeated electric field stimulation (EFS). The EFS response was only inhibited by high concentrations of atropine (.gtoreq. 10-5 M). In mucosa-submucosa preparations 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) was more effective than telenzepine whereas pirenzepine was ineffective. Pirenzepine inhibited the EFS response in mucosa preparations as did telenzepine and 4-DAMP. It was not possible to differentiate between the muscarinic receptors involved in the different parts of the enteric nervous system on the basis of our results.

Harvard-Zitierstil: Diener, M., Knobloch, S., Bridges, R., Keilmann, T. and Rummel, W. (1989) Cholinergic-mediated secretion in the rat colon: neuronal and epithelial muscarinic responses, European Journal of Pharmacology, 168(2), pp. 219-229. https://doi.org/10.1016/0014-2999(89)90568-2

APA-Zitierstil: Diener, M., Knobloch, S., Bridges, R., Keilmann, T., & Rummel, W. (1989). Cholinergic-mediated secretion in the rat colon: neuronal and epithelial muscarinic responses. European Journal of Pharmacology. 168(2), 219-229. https://doi.org/10.1016/0014-2999(89)90568-2