Phospholipase C-induced anion secretion and its interaction with carbachol in the rat colonie mucosa - Research portal of Justus Liebig University Giessen:

Journal article

Phospholipase C-induced anion secretion and its interaction with carbachol in the rat colonie mucosa

Authors list: Diener; Martin; Eglème, Cécile; Rummel, Walter

Publication year: 1991

Pages: 267-276

Journal: European Journal of Pharmacology

Volume number: 200

Issue number: 2-3

ISSN: 0014-2999

DOI Link: https://doi.org/10.1016/0014-2999(91)90581-A

Publisher: Elsevier

Abstract:
Phospholipase C (PLC) from clostridium perfringens induced a biphasic increase in short-circuit current (Isc) in the rat colon. The Isc rose rapidly to a transient peak, before it increased again to a plateau lasting for several hours. Ion replacement experiments and sensitivity to furosemide or a Cl- channel blocker indicated that PLC induced Cl- secretion. The first peak was suppressed by indomethacin, indicating mediation by prostaglandins. In contrast, the second phase was only partially sensitive to the cyclooxygenase blocker. The long-time action of PLC was dependent on intra- and extracellular Ca2+, although PLC did not induce an increase in the intracellular Ca2+ concentration of the enterocytes. The effect of PLC was blocked by the protein kinase inhibitor, staurosporine. Carbachol, when added during the second phase of the PLC response, induced a 'paradox' change in Isc: a rapid, transient increase in Isc was followed by a long-lasting decrease. This inhibition of the PLC response was more pronounced after elevation of the external Ca2+ concentration. A Ca2+ ionophore, ionomycin, and a Ca2+ channel activator, BAY K 8644, also inhibited the PLC response. The results suggest dual dependence of the action of PLC on the intracellular Ca2+ concentration.

Authors/Editors

- Uni.-Prof. Dr. Martin Heinz Diener

Citation Styles

Harvard Citation style: Diener, Martin, Eglème, C. and Rummel, W. (1991) Phospholipase C-induced anion secretion and its interaction with carbachol in the rat colonie mucosa, European Journal of Pharmacology, 200(2-3), pp. 267-276. https://doi.org/10.1016/0014-2999(91)90581-A

APA Citation style: Diener, Martin, Eglème, C., & Rummel, W. (1991). Phospholipase C-induced anion secretion and its interaction with carbachol in the rat colonie mucosa. European Journal of Pharmacology. 200(2-3), 267-276. https://doi.org/10.1016/0014-2999(91)90581-A