Journal article

Publication year: 2014

Journal: PLoS ONE

Volume number: 9

Issue number: 6

ISSN: 1932-6203

DOI Link: https://doi.org/10.1371/journal.pone.0100997

Publisher: Public Library of Science

Abstract: 
To identify specific genes determining the initiation and formation of adventitious roots (AR), a microarray-based transcriptome analysis in the stem base of the cuttings of Petunia hybrida (line W115) was conducted. A microarray carrying 24,816 unique, non-redundant annotated sequences was hybridized to probes derived from different stages of AR formation. After exclusion of wound-responsive and root-regulated genes, 1,354 of them were identified which were significantly and specifically induced during various phases of AR formation. Based on a recent physiological model distinguishing three metabolic phases in AR formation, the present paper focuses on the response of genes related to particular metabolic pathways. Key genes involved in primary carbohydrate metabolism such as those mediating apoplastic sucrose unloading were induced at the early sink establishment phase of AR formation. Transcriptome changes also pointed to a possible role of trehalose metabolism and SnRK1 (sucrose non-fermenting 1-related protein kinase) in sugar sensing during this early step of AR formation. Symplastic sucrose unloading and nucleotide biosynthesis were the major processes induced during the later recovery and maintenance phases. Moreover, transcripts involved in peroxisomal beta-oxidation were up-regulated during different phases of AR formation. In addition to metabolic pathways, the analysis revealed the activation of cell division at the two later phases and in particular the induction of G1-specific genes in the maintenance phase. Furthermore, results point towards a specific demand for certain mineral nutrients starting in the recovery phase.

Harvard Citation style: Ahkami, A., Scholz, U., Steuernagel, B., Strickert, M., Haensch, K., Druege, U., et al. (2014) Comprehensive Transcriptome Analysis Unravels the Existence of Crucial Genes Regulating Primary Metabolism during Adventitious Root Formation in Petunia hybrida, PLoS ONE, 9(6), Article e100997. https://doi.org/10.1371/journal.pone.0100997

APA Citation style: Ahkami, A., Scholz, U., Steuernagel, B., Strickert, M., Haensch, K., Druege, U., Reinhardt, D., Nouri, E., von Wiren, N., Franken, P., & Hajirezaei, M. (2014). Comprehensive Transcriptome Analysis Unravels the Existence of Crucial Genes Regulating Primary Metabolism during Adventitious Root Formation in Petunia hybrida. PLoS ONE. 9(6), Article e100997. https://doi.org/10.1371/journal.pone.0100997