Methods for the study of ionic currents and Ca2+-signals in isolated colonic crypts - Research portal of Justus Liebig University Giessen:

Journal article

Methods for the study of ionic currents and Ca2+-signals in isolated colonic crypts

Authors list: Schultheiss, G; Lan Kocks, S; Diener, M

Publication year: 2002

Pages: 70-78

Journal: Biological Procedures Online

Volume number: 3

ISSN: 1480-9222

DOI Link: https://doi.org/10.1251/bpo25

Publisher: BioMed Central

Abstract:
Isolated epithelial cells from intestinal mucosae are a suitable object for the study of the regulation of ion transport in the gut. This regulation possesses a great importance for human and veterinary medicine, as diarrheal diseases, which often are caused by an inadequate activation of intestinal anion secretion, are one of the major lethal diseases of children or young animals. The aim of this paper is to describe a method for the isolation of intact colonic crypts, e.g. for the subsequent investigation of the regulation of anion secretion by the intracellular second messenger, Ca(2+) using electrophysiological and imaging techniques.

Authors/Editors

- Uni.-Prof. Dr. Martin Heinz Diener

Citation Styles

Harvard Citation style: Schultheiss, G., Lan Kocks, S. and Diener, M. (2002) Methods for the study of ionic currents and Ca2+-signals in isolated colonic crypts, Biological Procedures Online, 3, pp. 70-78. https://doi.org/10.1251/bpo25

APA Citation style: Schultheiss, G., Lan Kocks, S., & Diener, M. (2002). Methods for the study of ionic currents and Ca2+-signals in isolated colonic crypts. Biological Procedures Online. 3, 70-78. https://doi.org/10.1251/bpo25