Journalartikel
Autorenliste: Lueck, Stefanie; Kreszies, Tino; Strickert, Marc; Schweizer, Patrick; Kuhlmann, Markus; Douchkov, Dimitar
Jahr der Veröffentlichung: 2019
Zeitschrift: Frontiers in Plant Science
Bandnummer: 10
ISSN: 1664-462X
Open Access Status: Gold
DOI Link: https://doi.org/10.3389/fpls.2019.01023
Verlag: Frontiers Media
Abstract:
RNA interference (RNAi) is a technique used for transgene-mediated gene silencing based on the mechanism of posttranscriptional gene silencing (PTGS). PTGS is an ubiquitous basic biological phenomenon involved in the regulation of transcript abundance and plants' immune response to viruses. PTGS also mediates genomic stability by silencing of retroelements. RNAi has become an important research tool for studying gene function by strong and selective suppression of target genes. Here, we present si-Fi, a software tool for design optimization of RNAi constructs necessary for specific target gene knockdown. It offers efficiency prediction of RNAi sequences and off-target search, required for the practical application of RNAi. si-Fi is an open-source (CC BY-SA license) desktop software that works in Microsoft Windows environment and can use custom sequence databases in standard FASTA format.
Zitierstile
Harvard-Zitierstil: Lueck, S., Kreszies, T., Strickert, M., Schweizer, P., Kuhlmann, M. and Douchkov, D. (2019) siRNA-Finder (si-Fi) Software for RNAi-Target Design and Off-Target Prediction, Frontiers in Plant Science, 10, Article 1023. https://doi.org/10.3389/fpls.2019.01023
APA-Zitierstil: Lueck, S., Kreszies, T., Strickert, M., Schweizer, P., Kuhlmann, M., & Douchkov, D. (2019). siRNA-Finder (si-Fi) Software for RNAi-Target Design and Off-Target Prediction. Frontiers in Plant Science. 10, Article 1023. https://doi.org/10.3389/fpls.2019.01023
Schlagwörter
FUNCTIONAL GENOMICS; INTERFERENCE; Off-target; posttranscriptional gene silencing; RNAi design; RNAi efficiency prediction; RNA interface; si-Fi
