Fast Ca2+-induced potentiation of heat-activated ionic currents requires cAMP/PKA signaling and functional AKAP anchoring - Research portal of Justus Liebig University Giessen:

Journal article

Fast Ca2+-induced potentiation of heat-activated ionic currents requires cAMP/PKA signaling and functional AKAP anchoring

Authors list: Distler, C; Rathee, PK; Lips, KS; Obreja, O; Neuhuber, W; Kress, M

Publication year: 2003

Pages: 2499-2505

Journal: Journal of Neurophysiology

Volume number: 89

Issue number: 5

ISSN: 0022-3077

DOI Link: https://doi.org/10.1152/jn.00713.2002

Publisher: American Physiological Society

Abstract:

Calcium influx and the resulting increase in intracellular calcium concentration ([Ca2+](i)) can induce enhanced sensitivity to temperature increases in nociceptive neurons. This sensitization accounts for heat hyperalgesia that is regularly observed following the activation of excitatory inward currents by pain-producing mediators. Here we show that rat sensory neurons express calcium-dependent adenylyl cyclases (AC) using RT-PCR and nonradioactive in situ hybridization. Ionomycin-induced rises in [Ca2+](i)-activated calcium-dependent AC and caused translocation of catalytic protein kinase A subunit. Elevation of [Ca2+](i) finally resulted in a significant potentiation of heat-activated currents and a drop in heat threshold. This was not prevented in the presence of suramin that nonspecifically uncouples G protein-dependent receptors. The sensitization was, however, inhibited when the specific PKA antagonist PKI14-22 was added to the pipette solution or when PKA coupling to A kinase anchoring protein ( AKAP) was disrupted with InCELLect StHt-31 uncoupling peptide. The results show that heat sensitization in nociceptive neurons can be induced by increases in [Ca2+](i) and requires PKA that is functionally coupled to the heat transducer, mostly likely vanilloid receptor VR-1. This calcium-dependent pathway can account for the sensitizing properties of many excitatory mediators that activate cationic membrane currents.

Authors/Editors

- apl.-Prof. PD Dr. Katrin Susanne Lips

Citation Styles

Harvard Citation style: Distler, C., Rathee, P., Lips, K., Obreja, O., Neuhuber, W. and Kress, M. (2003) Fast Ca2+-induced potentiation of heat-activated ionic currents requires cAMP/PKA signaling and functional AKAP anchoring, Journal of Neurophysiology, 89(5), pp. 2499-2505. https://doi.org/10.1152/jn.00713.2002

APA Citation style: Distler, C., Rathee, P., Lips, K., Obreja, O., Neuhuber, W., & Kress, M. (2003). Fast Ca2+-induced potentiation of heat-activated ionic currents requires cAMP/PKA signaling and functional AKAP anchoring. Journal of Neurophysiology. 89(5), 2499-2505. https://doi.org/10.1152/jn.00713.2002