Journal article

Publication year: 2014

Pages: 4518-4524

Journal: Acta Biomaterialia

Volume number: 10

Issue number: 10

ISSN: 1742-7061

eISSN: 1878-7568

DOI Link: https://doi.org/10.1016/j.actbio.2014.06.013

Publisher: Elsevier

Abstract: 
New strategies to decrease infection rates in cementless arthroplasty are needed, especially in the context of the growing incidence of methicillin-resistant Staphylococcus aureus (MRSA) infections. The purpose of this study was to investigate the antimicrobial activity of a rifampicin fosfomycin coating against methicillin-sensitive Staphylococcus aureus (MSSA) and MRSA in a rabbit infection prophylaxis model. Uncoated or rifampicin fosfomycin-coated K-wires were inserted into the intramedullary canal of the tibia in rabbits and contaminated with an inoculation dose of 105 or 106 colony-forming units of MSSA EDCC 5055 in study 1 and MRSA T6625930 in study 2, respectively. After 28 days the animals were killed and clinical, histological and microbiological assessment, including pulse-field gel electrophoresis, was conducted. Positive culture growth in agar plate testing and/or clinical signs and/or histological signs were defined positive for infection. Statistical evaluation was performed using Fisher's exact test. Both studies showed a statistically significant reduction of infection rates for rifampicin fosfomycin-coated implants compared to uncoated K-wires (P = 0.015). In both studies none of the 12 animals that were treated with a rifampicin fosfomycin-coated implant showed clinical signs of infection or a positive agar plate testing result. In both studies, one animal of the coating group showed the presence of sporadic bacteria with concomitant inflammatory signs in histology. The control groups in both studies exhibited an infection rate of 100% with clear clinical signs of infection and positive culture growth in all animals. In summary, the rifampicin fosfomycin-coating showed excellent antimicrobial activity against both MSSA and MRSA, and therefore warrants further clinical testing. (C) 2014 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Harvard Citation style: Alt, V., Kirchhof, K., Seim, F., Hrubesch, I., Lips, K., Mannel, H., et al. (2014) Rifampicin-fosfomycin coating for cementless endoprostheses: Antimicrobial effects against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA), Acta Biomaterialia, 10(10), pp. 4518-4524. https://doi.org/10.1016/j.actbio.2014.06.013

APA Citation style: Alt, V., Kirchhof, K., Seim, F., Hrubesch, I., Lips, K., Mannel, H., Domann, E., & Schnettler, R. (2014). Rifampicin-fosfomycin coating for cementless endoprostheses: Antimicrobial effects against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Acta Biomaterialia. 10(10), 4518-4524. https://doi.org/10.1016/j.actbio.2014.06.013