Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs - Research portal of Justus Liebig University Giessen:

Journal article

Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs

Authors list: Jakob, Viktor; Schreiner, Alexander; Tikkanen, Ritva; Starzinski-Powitz, Anna

Publication year: 2006

Pages: 3397-3408

Journal: Molecular Biology of the Cell

Volume number: 17

Issue number: 8

ISSN: 1059-1524

DOI Link: https://doi.org/10.1091/mbc.E05-11-1034

Publisher: American Society for Cell Biology

Abstract:
We recently identified transmembrane protein shrew-1 and showed that it is able to target to adherens junctions in polarized epithelial cells. This suggested shrew-1 possesses specific basolateral sorting motifs, which we analyzed by mutational analysis. Systematic mutation of amino acids in putative sorting signals in the cytoplasmic domain of shrew-1 revealed three tyrosines and a dileucine motif necessary for basolateral sorting. Substitution of these amino acids leads to apical localization of shrew-1. By applying tannic acid to either the apical or basolateral part of polarized epithelial cells, thereby blocking vesicle fusion with the plasma membrane, we obtained evidence that the apically localized mutants were primarily targeted to the basolateral membrane and were then redistributed to the apical domain. Further support for a postendocytic sorting mechanism of shrew-1 was obtained by demonstrating that mu 1B, a subunit of the epithelial cell-specific adaptor complex AP-1B, interacts with shrew-1. In conclusion, our data provide evidence for a scenario where shrew-1 is primarily delivered to the basolateral membrane by a so far unknown mechanism. Once there, adaptor protein complex AP-1B is involved in retaining shrew-1 at the basolateral membrane by postendocytic sorting mechanisms.

Authors/Editors

- Uni.-Prof. Dr. Ritva Tikkanen

Citation Styles

Harvard Citation style: Jakob, V., Schreiner, A., Tikkanen, R. and Starzinski-Powitz, A. (2006) Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs, Molecular Biology of the Cell, 17(8), pp. 3397-3408. https://doi.org/10.1091/mbc.E05-11-1034

APA Citation style: Jakob, V., Schreiner, A., Tikkanen, R., & Starzinski-Powitz, A. (2006). Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs. Molecular Biology of the Cell. 17(8), 3397-3408. https://doi.org/10.1091/mbc.E05-11-1034