Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN - Research portal of Justus Liebig University Giessen:

Journal article

Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN

Authors list: Schilling, Kirstin; Opitz, Nils; Wiesenthal, Anja; Oess, Stefanie; Tikkanen, Ritva; Müller-Esterl, Werner; Icking, Ann

Publication year: 2006

Pages: 3870-3880

Journal: Molecular Biology of the Cell

Volume number: 17

Issue number: 9

ISSN: 1059-1524

eISSN: 1939-4586

DOI Link: https://doi.org/10.1091/mbc.E05-08-0709

Publisher: American Society for Cell Biology

Abstract:
Recently, we characterized a novel endothelial nitric-oxide synthase (eNOS)-interacting protein, NOSTRIN (for eNOS-trafficking inducer), which decreases eNOS activity upon overexpression and induces translocation of eNOS away from the plasma membrane. Here, we show that NOSTRIN directly binds to caveolin-1, a well-established inhibitor of eNOS. Because this interaction occurs between the N terminus of caveolin (positions 1-61) and the central domain of NOSTRIN (positions 323-434), it allows for independent binding of each of the two proteins to eNOS. Consistently, we were able to demonstrate the existence of a ternary complex of NOSTRIN, eNOS, and caveolin-1 in Chinese hamster ovary (CHO)-eNOS cells. In human umbilical vein endothelial cells (HUVECs), the ternary complex assembles at the plasma membrane upon confluence or thrombin stimulation. In CHO-eNOS cells, NOSTRIN-mediated translocation of eNOS involves caveolin in a process most likely representing caveolar trafficking. Accordingly, trafficking of NOSTRIN/eNOS/ caveolin is affected by altering the state of actin filaments or cholesterol levels in the plasma membrane. During caveolar trafficking, NOSTRIN functions as an adaptor to recruit mediators such as dynamin-2 essential for membrane fission. We propose that a ternary complex between NOSTRIN, caveolin-1, and eNOS mediates translocation of eNOS, with important implications for the activity and availability of eNOS in the cell.

Authors/Editors

- Uni.-Prof. Dr. Ritva Tikkanen

Citation Styles

Harvard Citation style: Schilling, K., Opitz, N., Wiesenthal, A., Oess, S., Tikkanen, R., Müller-Esterl, W., et al. (2006) Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN, Molecular Biology of the Cell, 17(9), pp. 3870-3880. https://doi.org/10.1091/mbc.E05-08-0709

APA Citation style: Schilling, K., Opitz, N., Wiesenthal, A., Oess, S., Tikkanen, R., Müller-Esterl, W., & Icking, A. (2006). Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN. Molecular Biology of the Cell. 17(9), 3870-3880. https://doi.org/10.1091/mbc.E05-08-0709