Journal article

Publication year: 2002

Pages: 154-159

Journal: Nature Cell Biology

Volume number: 4

Issue number: 2

ISSN: 1465-7392

eISSN: 1476-4679

DOI Link: https://doi.org/10.1038/ncb745

Publisher: Nature Research

Abstract: 

Adaptors are heterotetrameric complexes that mediate the incorporation of cargo into transport vesicles by interacting with sorting signals present in the cytosolic domain of transmembrane proteins. Four adaptors, AP-1 (beta1, gamma, mu1A or mu1B, sigma1), AP-2 (beta2, alpha, mu2, sigma2), AP-3 (beta3, delta, mu3, sigma3) or AP-4 (beta4, epsilonc, mu4, sigma4), have been characterized(1,2). AP1 and AP-3 mediate sorting events at the level of the TGN and/or endosomes, whereas AP-2 functions in endocytic clathrin coated vesicle formation; no function is known so far for AP-4. Here, we show that AP-4 can bind different types of cytosolic signals known to mediate basolateral transport in epithelial cells. Furthermore, in MDCK cells with depleted mu4 protein levels, several basolateral proteins are mis-sorted to the apical surface, showing that AP-4 participates in basolateral sorting in epithelial cells.

Harvard Citation style: Simmen, T., Höning, S., Icking, A., Tikkanen, R. and Hunziker, W. (2002) AP-4 binds basolateral signals and participates in basolateral sorting in epithelial MDCK cells, Nature Cell Biology, 4(2), pp. 154-159. https://doi.org/10.1038/ncb745

APA Citation style: Simmen, T., Höning, S., Icking, A., Tikkanen, R., & Hunziker, W. (2002). AP-4 binds basolateral signals and participates in basolateral sorting in epithelial MDCK cells. Nature Cell Biology. 4(2), 154-159. https://doi.org/10.1038/ncb745