Journal article

Publication year: 2000

Pages: 3289-3298

Journal: Molecular Biology of the Cell

Volume number: 11

Issue number: 10

ISSN: 1059-1524

eISSN: 1939-4586

DOI Link: https://doi.org/10.1091/mbc.11.10.3289

Publisher: American Society for Cell Biology

Abstract: 

Endobrevin/VAMP-8 is an R-SNARE localized to endosomes, but it is unknown in which intracellular fusion step it operates. Using subcellular fractionation and quantitative immunogold electron microscopy, we found that endobrevin/VAMP-8 is present on all membranes known to communicate with early endosomes, including the plasma membrane, clathrin-coated pits, late endosomes, and membranes of the trans-Golgi network. Affinity-purified antibodies that block the ability of endobrevin/VAMP-8 to form SNARE core complexes potently inhibit homotypic fusion of both early and late endosomes in vitro. Fab fragments were as active as intact immunoglobulin Gs. Recombinant endobrevin/VAMP-8 inhibited both fusion reactions with similar potency. We conclude that endobrevin/VAMP-8 operates as an R-SNARE in the homotypic fusion of early and late endosomes.

Harvard Citation style: Antonin, W., Holroyd, C., Tikkanen, R., Höning, S. and Jahn, R. (2000) The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes, Molecular Biology of the Cell, 11(10), pp. 3289-3298. https://doi.org/10.1091/mbc.11.10.3289

APA Citation style: Antonin, W., Holroyd, C., Tikkanen, R., Höning, S., & Jahn, R. (2000). The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes. Molecular Biology of the Cell. 11(10), 3289-3298. https://doi.org/10.1091/mbc.11.10.3289