Journal article

Publication year: 2018

Pages: 8856-8864

Journal: Analytical Chemistry

Volume number: 90

Issue number: 15

ISSN: 0003-2700

DOI Link: https://doi.org/10.1021/acs.analchem.8b00892

Publisher: American Chemical Society

Abstract: 
A method is described for high-resolution label-free molecular ToF-SIMS imaging of human bone tissue. To preserve the lipid content and the heterogeneous structure of osseous tissue, 4 mu m thick human bone sections were prepared via cryoembedding and tape-assisted cryosectioning, circumventing the application of organic solvents and a decalcification step. A protocol for comparative mass spectrometry imaging (MSI) on the same section was established for initial analysis with time-of-flight secondary ion mass spectrometry (TOF-SIMS) at a lateral resolution of 10 mu m to <500 nm, followed by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) Orbitrap MSI at a lateral resolution of 10 mu m. This procedure ultimately enabled MSI of lipids, providing the lateral localization of major lipid classes such as glycero-, glycerophospho-, and sphingolipids. Additionally, the applicability of the recently emerged Orbitrap-TOF-SIMS hybrid system was exemplarily examined and compared to the before-mentioned MSI methods.

Harvard Citation style: Schaepe, K., Bhandari, D., Werner, J., Henss, A., Pirkl, A., Kleine-Boymann, M., et al. (2018) Imaging of Lipids in Native Human Bone Sections Using TOF-Secondary Ion Mass Spectrometry, Atmospheric Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Orbitrap-Mass Spectrometry, and Orbitrap-Secondary Ion Mass Spectrometry, Analytical Chemistry, 90(15), pp. 8856-8864. https://doi.org/10.1021/acs.analchem.8b00892

APA Citation style: Schaepe, K., Bhandari, D., Werner, J., Henss, A., Pirkl, A., Kleine-Boymann, M., Rohnke, M., Wenisch, S., Neumann, E., Janek, J., & Spengler, B. (2018). Imaging of Lipids in Native Human Bone Sections Using TOF-Secondary Ion Mass Spectrometry, Atmospheric Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Orbitrap-Mass Spectrometry, and Orbitrap-Secondary Ion Mass Spectrometry. Analytical Chemistry. 90(15), 8856-8864. https://doi.org/10.1021/acs.analchem.8b00892