Sammelbandbeitrag

Erschienen in: Xenopus : Methods and Protocols

Herausgeberliste: Vleminckx, Kris

Jahr der Veröffentlichung: 2018

Seiten: 217-231

ISBN: 978-1-4939-8783-2

eISBN: 978-1-4939-8784-9

DOI Link: https://doi.org/10.1007/978-1-4939-8784-9_15

Serientitel: Methods in Molecular Biology

Serienzählung: 1865

Abstract: 

Electroporation is an efficient method of transferring charged macromolecules into living cells in order to study their morphology, function, and connectivity within neuronal networks. Labeling cells with fluorophore-coupled macromolecules can be used to trace projections of whole neuronal ensembles, as well as the fine morphology of single cells. Here, we present a protocol to visualize pre- and postsynaptic components of a sensory relay synapse in the brain, using the olfactory system of Xenopus laevis tadpoles as a model. We apply bulk electroporation to trace projections of receptor neurons from the nose to the brain, and single cell electroporation to visualize the morphology of their synaptic target cells, the mitral-tufted cells. Labeling the receptor neurons with a calcium-sensitive dye allows us to record stimulus-induced presynaptic input to the dendrites of the postsynaptic cells via functional calcium imaging.

Harvard-Zitierstil: Weiss, L., Offner, T., Hassenklöver, T. and Manzini, I. (2018) Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System., in Vleminckx, K. (ed.) Xenopus : Methods and Protocols. New York, NY: Humana Press, pp. 217-231. https://doi.org/10.1007/978-1-4939-8784-9_15

APA-Zitierstil: Weiss, L., Offner, T., Hassenklöver, T., & Manzini, I. (2018). Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System.. In Vleminckx, K. (Ed.), Xenopus : Methods and Protocols (pp. 217-231). Humana Press. https://doi.org/10.1007/978-1-4939-8784-9_15