Journal article

Publication year: 2019

Pages: 1682-1688

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 69

Issue number: 6

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.003376

Publisher: Microbiology Society

Abstract: 
Two slightly orange-pigmented, oxidase-positive bacterial strains (M1-83(T)and M2-116), isolated from horse blood collected during slaughter in Giessen, Germany, were studied in a polyphasic taxonomic approach. Cells of the isolates were coccoid and stained Gram-negative. The two strains shared identical 16S rRNA gene sequences but their genomic fingerprint patterns differed, indicating the genetic distinctiveness of the two strains. A comparison of the 16S rRNA gene sequence of strain M1-83(T)with sequences of the type strains of the most closely related Paracoccus species showed highest sequence similarities to Paracoccus acridae (98.2 %) and Paracoccus aerius (98.1 %). 16S rRNA gene sequence similarities to all other Paracoccus species were below 97.6%. The fatty acid profile of the two strains consisted mainly of the major fatty acids C-18:1 omega 7c and C-18:0, which is typical for the genus Paracoccus. The polyamine patterns of strain M1-83(T )contained major amounts of putrescine and spermidine. The major quinone was ubiquinone Q-10. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. The polar lipid profile was characterized by the major lipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and an unidentified glycolipid. DNA-DNA hybridization experiments between M1-83(T) and the type strains of P. acridae and P. aerius resulted in similarity values of 17 % (reciprocal, 60 %) and 23 % (reciprocal 30 %), respectively. DNA-DNA hybridization results together with the differentiating biochemical and chemotaxonomic properties showed that strain M1-83(T)represents a novel Paracoccus species, for which the name Paracoccus haematequi sp. nov. (type strain M1-83(T) =LMG 30633(T)=CIP 111624(T) =CCM 8857(T)), is proposed.

Harvard Citation style: Kämpfer, P., Busse, H., Galatis, H., Criscuolo, A., Clermont, D., Bizet, C., et al. (2019) Paracoccus haematequi sp. nov., isolated from horse blood, International Journal of Systematic and Evolutionary Microbiology, 69(6), pp. 1682-1688. https://doi.org/10.1099/ijsem.0.003376

APA Citation style: Kämpfer, P., Busse, H., Galatis, H., Criscuolo, A., Clermont, D., Bizet, C., & Glaeser, S. (2019). Paracoccus haematequi sp. nov., isolated from horse blood. International Journal of Systematic and Evolutionary Microbiology. 69(6), 1682-1688. https://doi.org/10.1099/ijsem.0.003376