Journal article

Publication year: 2018

Pages: 1210-1214

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 68

Issue number: 4

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.002652

Publisher: Microbiology Society

Abstract: 
A beige-pigmented bacterial strain, SB30-Chr27-3(T), isolated from a garden pond, was studied for its taxonomic position. Cells of the isolate were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence with the sequences of the type strains of the most closely related species showed that the strain belongs to the genus Comamonas and showed highest sequence similarities to the type strains of Comamonas jiangduensis (97.5 %), Comamonas aquatica (97.4 %) and Comamonas phosphati (97.3 %). The 16S rRNA gene sequence similarities to all other Comamonas species were below 97.0 %. The fatty acid profile of strain SB30-Chr27-3(T) consisted of the major fatty acids C-16 : 0, C(15 : 0)iso 2-OH/ C-16 : 1 omega 7c, C-18 : 1 omega 7c/C-18 : 1 omega 9c and, in a minor amount, C-10 : 0 3-OH. Major compounds in the polar lipid profile were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and diphosphatidylglycerol. The quinone system was exclusively composed of ubiquinone Q-8. The polyamine pattern contained the major compounds putrescine, cadaverine and 2-hydroxyputrescine. These data and the differentiating biochemical properties indicated that isolate SB30-CHR27-3(T) represents a novel species of the genus Comamonas, for which we propose the name Comamonas aquatilis sp. nov. with the type strain SB30-Chr27-3(T) (=CIP 111491(T)=CCM 8815(T)).

Harvard Citation style: Kämpfer, P., Busse, H., Baars, S., Wilharm, G. and Glaeser, S. (2018) Comamonas aquatilis sp nov., isolated from a garden pond, International Journal of Systematic and Evolutionary Microbiology, 68(4), pp. 1210-1214. https://doi.org/10.1099/ijsem.0.002652

APA Citation style: Kämpfer, P., Busse, H., Baars, S., Wilharm, G., & Glaeser, S. (2018). Comamonas aquatilis sp nov., isolated from a garden pond. International Journal of Systematic and Evolutionary Microbiology. 68(4), 1210-1214. https://doi.org/10.1099/ijsem.0.002652