Journal article

Publication year: 2018

Pages: 1037-1046

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 68

Issue number: 4

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.002616

Publisher: Microbiology Society

Abstract: 
A Gram-stain-negative, aerobic, non-endospore-forming organism, isolated from the rhizosphere sand of a coastal sand dune plant was studied for its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain YU-PRIM-29(T) was grouped within the genus Halomonas and was most closely related to Halomonas johnsoniae (97.5 %). The 16S rRNA gene sequence similarity to other Halomonas species was < 97.5 %. Strain YU-PRIM-29 T grew optimally at 28 degrees C (growth range, 10-36 degrees C), at a pH of 7-9 (growth range, pH 5.5-12.0) and in the presence of 0.5 to 5% (w/v) NaCl (growth up to 20% NaCl). The fatty acid profile from whole-cell hydrolysates supported the allocation of the strain to the genus Halomonas. The fatty acids C-18 : 1 omega 7c and C-16 : 0 were found as major compounds, followed by the hydroxylated fatty acid C-12 : 0 3-OH. The quinone system consisted predominantly of ubiquinone Q-9. The polar lipid profile was composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In the polyamine pattern, spermidine was the predominant compound. The DNA G+C content was 64.8 mol%. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain YU-PRIM-29(T) from its closest-related species. Hence, YU-PRIM-29(T) represents a new species of the genus Halomonas, for which we propose the name Halomonas malpeensis sp. nov., with YUPRIM-29(T) (= LMG 28855(T) = CCM 8737(T)) as the type strain.

Harvard Citation style: Kämpfer, P., Rekha, P., Busse, H., Arun, A., Priyanka, P. and Glaeser, S. (2018) Halomonas malpeensis sp nov., isolated from rhizosphere sand of a coastal sand dune plant, International Journal of Systematic and Evolutionary Microbiology, 68(4), pp. 1037-1046. https://doi.org/10.1099/ijsem.0.002616

APA Citation style: Kämpfer, P., Rekha, P., Busse, H., Arun, A., Priyanka, P., & Glaeser, S. (2018). Halomonas malpeensis sp nov., isolated from rhizosphere sand of a coastal sand dune plant. International Journal of Systematic and Evolutionary Microbiology. 68(4), 1037-1046. https://doi.org/10.1099/ijsem.0.002616