Journal article

Publication year: 2018

Pages: 2454-2461

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 68

Issue number: 8

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.002854

Publisher: Microbiology Society

Abstract: 
Five beige-pigmented, oxidase-negative bacterial isolates, 6331-17(T), 6332-17, 6333-17, 6334-17 and 9827-07, isolated either from a drinking water storage reservoir or drinking water in 2006 and 2017 in Germany, were examined in detail applying by a polyphasic taxonomic approach. Cells of the isolates were rod-shaped and Gram-stain-negative. Comparison of the 16S rRNA gene sequences of these five isolates showed highest sequence similarities to Lelliottia amnigena (99.98 %) and Lelliottia nimipressuralis (99.99 %). Multilocus sequence analyses based on concatenated partial rpoB, gyrB, infB and atpD sequences confirmed the clustering of these isolates with Lelliottia species, but also revealed a clear distinction to the closest related type strains. Analysis of the genome sequences of these isolates indicated >70% in silico DNA-DNA hybridization and high average nucleotide identities between strains Nevertheless, they showed only <70 and <95% similarity to the type strains of these two Lelliottia species. The fatty acid profiles of these isolates were very similar and consisted of the major fatty acids C-16:0,C- C(17:0)cyclo, C(15:0)iso 2-OH/C-16:1 omega 7c and C-18:1 omega 7c. In addition, physiological/ biochemical tests revealed high phenotypic similarity to each other. These cumulative data indicate that these isolates represent a novel Lelliottia species, for which the name Lelliottia aquatilis sp. nov. is proposed, with strain 6331-17(T) (=CCM 8846(T)=CIP 1 1 1 609(T)=LMG 30560(T)) as the type strain.

Harvard Citation style: Kämpfer, P., Glaeser, S., Packroff, G., Behringer, K., Exner, M., Chakraborty, T., et al. (2018) Lelliottia aquatilis sp nov., isolated from drinking water, International Journal of Systematic and Evolutionary Microbiology, 68(8), pp. 2454-2461. https://doi.org/10.1099/ijsem.0.002854

APA Citation style: Kämpfer, P., Glaeser, S., Packroff, G., Behringer, K., Exner, M., Chakraborty, T., Schmithausen, R., & Doijad, S. (2018). Lelliottia aquatilis sp nov., isolated from drinking water. International Journal of Systematic and Evolutionary Microbiology. 68(8), 2454-2461. https://doi.org/10.1099/ijsem.0.002854