Journal article

Publication year: 2018

Pages: 1560-1564

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 68

Issue number: 5

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.002702

Publisher: Microbiology Society

Abstract: 
A yellow-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain KSS165-70(T)) was isolated from a coolant lubricant emulsion. The 16S rRNA gene sequence analysis of strain KSS165-70(T) showed high sequence similarity to the type strains of Novosphingobium subterraneum (98.1 %), Novosphingobium lentum (97.9 %) and Novosphingobium taihuense (97.8 %). Sequence similarities to type strains of all other Novosphingobium species were below 97.5 %. Ubiquinone Q-10 was detected as the major respiratory quinone. The predominant fatty acid C-18 : 1 omega 7c and the typical 2-hydroxy fatty acid C-14 : 0 2-OH were detected. The polar lipid profile contained the major lipids diphosphatidylglycerol, phosphatedylethanolamine, sphingoglycolipid, phosphatidylcholine and two unidentified phospholipids. The polyamine pattern contained the major compound spermidine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, and ubiquinone, polar lipid and fatty acid composition revealed that strain KSS165-70(T) represents a new species of the genus Novosphingobium. For this reason, we propose the name Novosphingobium lubricantis sp. nov. with the type strain KSS165-70(T) (=CIP 111490(T) =CCM 8814(T)).

Harvard Citation style: Kämpfer, P., Busse, H. and Glaeser, S. (2018) Novosphingobium lubricantis sp nov., isolated from a coolant lubricant emulsion, International Journal of Systematic and Evolutionary Microbiology, 68(5), pp. 1560-1564. https://doi.org/10.1099/ijsem.0.002702

APA Citation style: Kämpfer, P., Busse, H., & Glaeser, S. (2018). Novosphingobium lubricantis sp nov., isolated from a coolant lubricant emulsion. International Journal of Systematic and Evolutionary Microbiology. 68(5), 1560-1564. https://doi.org/10.1099/ijsem.0.002702