Journal article

Publication year: 2017

Pages: 4956-4961

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 67

Issue number: 12

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.002357

Publisher: Microbiology Society

Abstract: 
A Gram-positive-staining, aerobic, non-endospore-forming bacterial strain (JJ-59(T)), isolated from a field-grown maize plant in Dunbar, Nebraska in 2014 was studied by a polyphasic approach. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-59(T) was shown to be a member of the genus Paenibacillus, most closely related to the type strains of Paenibacillus aceris (98.6% 16S rRNA gene sequence similarity) and Paenibacillus chondroitinus (97.8 %). For all other type strains of species of the genus Paenibacillus lower 16S rRNA gene sequence similarities were obtained. DNA-DNA hybridization values of strain JJ-59(T) to the type strains of P. aceris and P. chondroitinus were 26% (reciprocal, 59 %) and 52% (reciprocal, 59 %), respectively. Chemotaxonomic characteristics such as the presence of meso-diaminopimelic acid in the peptidoglycan, the major quinone MK-7 and spermidine as the major polyamine were in agreement with the characteristics of the genus Paenibacillus. Strain JJ-59(T) shared with its next related species P. aceris the major lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminophospholipid, but the presence/absence of certain lipids was clearly distinguishable. Major fatty acids of strain JJ-59(T) were anteiso-C15 : 0, isoC15 : 0 and iso-C16 : 0, and the genomic G+C content is 47.2 mol%. Physiological and biochemical characteristics of strain JJ-59(T) were clearly different from the most closely related species of the genus Paenibacillus. Thus, strain JJ-59(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus nebraskensis sp. nov. is proposed, with JJ-59 T (= DSM 103623(T) = CIP 111179(T) = LMG 29764(T)) as the type strain.

Harvard Citation style: Kämpfer, P., Busse, H., McInroy, J., Hu, C., Kloepper, J. and Glaeser, S. (2017) Paenibacillus nebraskensis sp nov., isolated from the root surface of field-grown maize, International Journal of Systematic and Evolutionary Microbiology, 67(12), pp. 4956-4961. https://doi.org/10.1099/ijsem.0.002357

APA Citation style: Kämpfer, P., Busse, H., McInroy, J., Hu, C., Kloepper, J., & Glaeser, S. (2017). Paenibacillus nebraskensis sp nov., isolated from the root surface of field-grown maize. International Journal of Systematic and Evolutionary Microbiology. 67(12), 4956-4961. https://doi.org/10.1099/ijsem.0.002357