Conference paper

Appeared in: IBIC 2012, 3rd International Conference on Industrial Biotechnology

Editor list: Bardone, Enrico

Publication year: 2012

Pages: 349-354

ISBN: 978-88-95608-18-1

DOI Link: https://doi.org/10.3303/CET1227059

Conference: 3rd International Conference on Industrial Biotechnology (IBIC 2012)

Title of series: Chemical engineering transactions

Number in series: 27

Abstract: 

In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

Harvard Citation style: Prinz, A., Zeiner, T., Vössing, T., Schüttmann, I., Zorn, H. and Gorak, A. (2012) Experimental Investigation of Laccase Purification Using Aqueous Two-Phase Extraction, in Bardone, E. (ed.) IBIC 2012, 3rd International Conference on Industrial Biotechnology. Milano: AIDIC. pp. 349-354. https://doi.org/10.3303/CET1227059

APA Citation style: Prinz, A., Zeiner, T., Vössing, T., Schüttmann, I., Zorn, H., & Gorak, A. (2012). Experimental Investigation of Laccase Purification Using Aqueous Two-Phase Extraction. In Bardone, E. (Ed.), IBIC 2012, 3rd International Conference on Industrial Biotechnology. (pp. 349-354). AIDIC. https://doi.org/10.3303/CET1227059