Journalartikel

Jahr der Veröffentlichung: 2009

Seiten: 427-435

Zeitschrift: Development Genes and Evolution

Bandnummer: 219

Heftnummer: 8

ISSN: 0949-944X

DOI Link: https://doi.org/10.1007/s00427-009-0301-0

Verlag: Springer

Abstract: 
The genes encoding the closely related zinc finger transcription factors Buttonhead (Btd) and D-Sp1 are expressed in the developing limb primordia of Drosophila melanogaster and are required for normal growth of the legs. The D-Sp1 homolog of the red flour beetle Tribolium castaneum, Sp8 (appropriately termed Sp8/9), is also required for the proper growth of the leg segments. Here we report on the isolation and functional study of the Sp8/9 gene from the milkweed bug Oncopeltus fasciatus. We show that Sp8/9 is expressed in the developing appendages throughout development and that the downregulation of Sp8/9 via RNAi leads to antennae, rostrum, and legs with shortened and fused segments. This supports a conserved role of Sp8/9 in allometric leg segment growth. However, all leg segments including the claws are present and the expression of the leg genes Distal-less, dachshund, and homothorax are proportionally normal, thus providing no evidence for a role of Sp8/9 in appendage specification.

Harvard-Zitierstil: Schaeper, N., Prpic, N. and Wimmer, E. (2009) A conserved function of the zinc finger transcription factor Sp8/9 in allometric appendage growth in the milkweed bug Oncopeltus fasciatus, Development Genes and Evolution, 219(8), pp. 427-435. https://doi.org/10.1007/s00427-009-0301-0

APA-Zitierstil: Schaeper, N., Prpic, N., & Wimmer, E. (2009). A conserved function of the zinc finger transcription factor Sp8/9 in allometric appendage growth in the milkweed bug Oncopeltus fasciatus. Development Genes and Evolution. 219(8), 427-435. https://doi.org/10.1007/s00427-009-0301-0