Journal article

Publication year: 2008

Journal: Cold Spring Harbor protocols

Volume number: 3

Issue number: 10

DOI Link: https://doi.org/10.1101/pdb.prot5068

Publisher: Cold Spring Harbor

Abstract: 

The spider Cupiennius salei, commonly known as the American wandering spider, is a particularly useful laboratory model for embryological studies because
of the availability of tools to study and manipulate its embryonic development. Cupiennius
is used to study axis formation, segmentation, appendage development,
neurogenesis, and silk production. These studies contribute
to our understanding of the evolution of these
processes, but they also help us to understand the origin and
diversification
of evolutionary novelties. Comparisons between
spiders and insects can show the degree of conservation and divergence
of developmental
mechanisms during arthropod evolution. Any
embryological feature conserved between spiders and insects is likely to
represent
an ancestral feature for arthropods. Comparative
molecular embryological work in insects and spiders should eventually
allow
us to define a molecular archetype for the phylum
Arthropoda. This in itself will be a necessary cornerstone for comparing
the different metazoan phyla, including chordates.
This protocol describes the detection of transcripts in fixed C. salei embryos using whole-mount in situ hybridization.

Harvard Citation style: Prpic, N., Schoppmeier, M. and Damen, W. (2008) Whole-Mount In Situ Hybridization of Spider Embryos, Cold Spring Harbor protocols, 3(10). https://doi.org/10.1101/pdb.prot5068

APA Citation style: Prpic, N., Schoppmeier, M., & Damen, W. (2008). Whole-Mount In Situ Hybridization of Spider Embryos. Cold Spring Harbor protocols. 3(10). https://doi.org/10.1101/pdb.prot5068