Journalartikel

Jahr der Veröffentlichung: 2008

Zeitschrift: Cold Spring Harbor protocols

Bandnummer: 3

Heftnummer: 10

DOI Link: https://doi.org/10.1101/pdb.prot5070

Verlag: Cold Spring Harbor

Abstract: 
The spider Cupiennius salei, commonly known as the American wandering spider, is a particularly useful laboratory model for embryological studies because
of the availability of tools to study and manipulate its embryonic development. Cupiennius
is used to study axis formation, segmentation, appendage development,
neurogenesis, and silk production. These studies contribute
to our understanding of the evolution of these
processes, but they also help us to understand the origin and
diversification
of evolutionary novelties. Comparisons between
spiders and insects can show the degree of conservation and divergence
of developmental
mechanisms during arthropod evolution. Any
embryological feature conserved between spiders and insects is likely to
represent
an ancestral feature for arthropods. Comparative
molecular embryological work in insects and spiders should eventually
allow
us to define a molecular archetype for the phylum
Arthropoda. This in itself will be a necessary cornerstone for comparing
the different metazoan phyla, including chordates.
The discovery of RNA interference (RNAi), in which double-stranded RNA
(dsRNA) suppresses the translation of homologous
mRNA, has had a huge impact on evolutionary developmental biology by
enabling
the analysis of loss-of-function phenotypes in
organisms in which classical genetic analysis is laborious or not
possible.
This protocol describes the application of RNAi to
embryos of the spider C. salei.

Harvard-Zitierstil: Prpic, N., Schoppmeier, M. and Damen, W. (2008) Gene Silencing via Embryonic RNAi in Spider Embryos, Cold Spring Harbor protocols, 3(10). https://doi.org/10.1101/pdb.prot5070

APA-Zitierstil: Prpic, N., Schoppmeier, M., & Damen, W. (2008). Gene Silencing via Embryonic RNAi in Spider Embryos. Cold Spring Harbor protocols. 3(10). https://doi.org/10.1101/pdb.prot5070