Journal article

Publication year: 2008

Journal: Cold Spring Harbor protocols

Volume number: 3

Issue number: 10

DOI Link: https://doi.org/10.1101/pdb.prot5071

Publisher: Cold Spring Harbor

Abstract: 
The spider Cupiennius salei, commonly known as the American wandering spider, is particularly useful for embryological studies because of the availability
of tools to study and manipulate its embryonic development. Cupiennius
is used to study axis formation, segmentation, appendage development,
neurogenesis, and silk production. These studies contribute
to our understanding of the evolution of these
processes, but they also help us to understand the origin and
diversification
of evolutionary novelties. Comparisons between
spiders and insects can show the degree of conservation and divergence
of developmental
mechanisms during arthropod evolution. Any
embryological feature conserved between spiders and insects is likely to
represent
an ancestral feature for arthropods. Comparative
molecular embryological work in insects and spiders should eventually
allow
us to define a molecular archetype for the phylum
Arthropoda. This will be a necessary cornerstone for comparing the
different
metazoan phyla, including chordates. This protocol
describes the detection of proliferating cells in whole-mount Cupiennius
embryos. When labeled nucleotides are introduced into mitotically
dividing cells, these cells incorporate the labels into
the newly synthesized DNA. Thus, only cells that
have synthesized DNA after the addition of the label will be detected.
This
protocol uses 5-bromo-2′-deoxy-uridine (BrdU) as a
label that is subsequently detected by immunocytochemistry. BrdU
labeling
is a relatively easy way to detect cells that have
recently synthesized DNA. The main advantage of this technique is that
the label accumulates over time and, by varying the
incubation time before fixation, an increasingly cumulative picture of
cell proliferation activity can be obtained.

Harvard Citation style: Prpic, N., Schoppmeier, M. and Damen, W. (2008) Detection of Cell Proliferation in Spider Embryos Using BrdU Labeling, Cold Spring Harbor protocols, 3(10). https://doi.org/10.1101/pdb.prot5071

APA Citation style: Prpic, N., Schoppmeier, M., & Damen, W. (2008). Detection of Cell Proliferation in Spider Embryos Using BrdU Labeling. Cold Spring Harbor protocols. 3(10). https://doi.org/10.1101/pdb.prot5071