Journalartikel

Jahr der Veröffentlichung: 2008

Zeitschrift: Cold Spring Harbor protocols

Bandnummer: 3

Heftnummer: 10

DOI Link: https://doi.org/10.1101/pdb.prot5072

Verlag: Cold Spring Harbor

Abstract: 
The spider Cupiennius salei, commonly known as the American wandering spider, is a particularly useful laboratory model for embryological studies because
of the availability of tools to study and manipulate its embryonic development. Cupiennius
is used to study axis formation, segmentation, appendage development,
neurogenesis, and silk production. These studies contribute
to our understanding of the evolution of these
processes, but they also help us to understand the origin and
diversification
of evolutionary novelties. Comparisons between
spiders and insects can show the degree of conservation and divergence
of developmental
mechanisms during arthropod evolution. Any
embryological feature conserved between spiders and insects is likely to
represent
an ancestral feature for arthropods. Comparative
molecular embryological work in insects and spiders should eventually
allow
us to define a molecular archetype for the phylum
Arthropoda. This in itself will be a necessary cornerstone for comparing
the different metazoan phyla, including chordates.
Spider embryos can be studied as whole mounts under the dissection
microscope.
Alternatively, the embryos can be dissected and
observed under the compound microscope. Preparing and dissecting spider
embryos
for compound microscopy is difficult due to the
high amount of yolk, which makes the embryos very fragile. The following
protocol
describes how to make the necessary tools, then use
them to obtain good preparations. Not all embryonic stages can be
dissected
and prepared; very young stages can only be
examined as whole mounts.

Harvard-Zitierstil: Prpic, N., Schoppmeier, M. and Damen, W. (2008) Dissecting Spider Embryos for Light Microscopy, Cold Spring Harbor protocols, 3(10). https://doi.org/10.1101/pdb.prot5072

APA-Zitierstil: Prpic, N., Schoppmeier, M., & Damen, W. (2008). Dissecting Spider Embryos for Light Microscopy. Cold Spring Harbor protocols. 3(10). https://doi.org/10.1101/pdb.prot5072