Human placenta thioredoxin reductase - Isolation of the selenoenzyme, steady state kinetics, and inhibition by therapeutic gold compounds - Research portal of Justus Liebig University Giessen:

Journal article

Human placenta thioredoxin reductase - Isolation of the selenoenzyme, steady state kinetics, and inhibition by therapeutic gold compounds

Authors list: Gromer, S; Arscott, LD; Williams, CH; Schirmer, RH; Becker, K

Publication year: 1998

Pages: 20096-20101

Journal: Journal of Biological Chemistry

Volume number: 273

Issue number: 32

ISSN: 0021-9258

eISSN: 1083-351X

DOI Link: https://doi.org/10.1074/jbc.273.32.20096

Publisher: Elsevier

Abstract:
Human thioredoxin reductase is a pyridine nucleotide-disulfide oxidoreductase closely related to glutathione reductase but differing from the latter in having a Cys-SeCys (selenocysteine) sequence as an additional redox center. Because selenoproteins cannot be expressed yet in heterologous systems, we optimized the purification of the protein from placenta with respect to final yield (1-2 mg from one placenta), specific activity (42 units/mg), and selenium content (0.94 +/- 0.03 mol/mol subunit). The steady state kinetics showed that the enzyme operates by a ping-pong mechanism; the value of k(cat) was 3330 +/- 882 min(-1), and the K-m values were 18 mu M for NADPH and 25 mu M for Escherichia coli thioredoxin. The activation energy of the reaction was found to be 53.2 kJ/mol, which allows comparisons of the steady state data with previous pre-steady state measurements. In its physiological, NADPH-rednced form, the enzyme is strongly inhibited by organic gold compounds that are widely used in the treatment of rheumatoid arthritis; for auranofin, the K-i was 4 nM when measured in the presence of 50 mu M thioredoxin. At 1000-fold higher concentrations, that is at micromolar levels, the drugs also inhibited human glutathione reductase and the selenoenzyme glutathione peroxidase.

Citation Styles

Harvard Citation style: Gromer, S., Arscott, L., Williams, C., Schirmer, R. and Becker, K. (1998) Human placenta thioredoxin reductase - Isolation of the selenoenzyme, steady state kinetics, and inhibition by therapeutic gold compounds, Journal of Biological Chemistry, 273(32), pp. 20096-20101. https://doi.org/10.1074/jbc.273.32.20096

APA Citation style: Gromer, S., Arscott, L., Williams, C., Schirmer, R., & Becker, K. (1998). Human placenta thioredoxin reductase - Isolation of the selenoenzyme, steady state kinetics, and inhibition by therapeutic gold compounds. Journal of Biological Chemistry. 273(32), 20096-20101. https://doi.org/10.1074/jbc.273.32.20096