Journal article

Publication year: 2006

Pages: 147-152

Journal: Journal of Chromatography B

Volume number: 837

Issue number: 1-2

ISSN: 1570-0232

DOI Link: https://doi.org/10.1016/j.jchromb.2006.04.012

Publisher: Elsevier

Abstract: 
Atmospheric pressure photoionisation (APPI) was used in combination with silver-ion (Ag(+))-HPLC for detection of (conjugated) fatty acid methyl esters (FAME) by tandem-mass spectrometry. APPI-MS of methyl esters of conjugated linoleic acid showed an increase in signal-to-noise ratio by a factor of 40 compared to atmospheric pressure chemical ionization in the positive mode. It was possible to identify double bond position, configuration and chain length of FAME based on chromatographic separation and mass detection. The developed LC-MS method is useful for the analysis of CLA elongation and beta-oxidation products, especially with trans,trans-configuration, which are difficult to analyze by conventional GC-MS techniques.

Harvard Citation style: Müller, A., Mickel, M., Geyer, R., Ringseis, R., Eder, K. and Steinhart, H. (2006) Identification of conjugated linoleic acid elongation and β-oxidation products by coupled silver-ion HPLC APPI-MS, Journal of Chromatography B, 837(1-2), pp. 147-152. https://doi.org/10.1016/j.jchromb.2006.04.012

APA Citation style: Müller, A., Mickel, M., Geyer, R., Ringseis, R., Eder, K., & Steinhart, H. (2006). Identification of conjugated linoleic acid elongation and β-oxidation products by coupled silver-ion HPLC APPI-MS. Journal of Chromatography B. 837(1-2), 147-152. https://doi.org/10.1016/j.jchromb.2006.04.012