Journal article

Publication year: 1999

Pages: 135-138

Journal: FEBS Letters

Volume number: 447

Issue number: 2-3

ISSN: 0014-5793

DOI Link: https://doi.org/10.1016/S0014-5793(99)00296-3

Publisher: Wiley

Abstract: 
The flavoenzyme glutathione reductase catalyses electron transfer reactions between two major intracellular redox buffers, namely the NADPH/NADP(+) couple and the 2 glutathione/glutathione disulfide couple, On this account, microcrystals of the enzyme were tested as redox probes of intracellular compartments. For introducing protein crystals into human fibroblasts, different methods (microinjection, particle bombardment and optical tweezers) were explored and compared. When glutathione reductase crystals are present in a cytosolic environment, the transition of the yellow E-OX form to the orange-red 2-electron reduced charge transfer form, EH2, is observed. Taking into account the midpoint potential of the E-OX/ EH2 couple, the redox potential of the cytosol was found to be <-270 mV at pH 7.4 and 37 degrees C. As a general conclusion, competent proteins in crystalline - that is signal-amplifying form are promising probes for studying intracellular events. (C) 1999 Federation of European Biochemical Societies.

Harvard Citation style: Keese, M., Saffrich, R., Dandekar, T., Becker, K. and Schirmer, R. (1999) Microinjected glutathione reductase crystals as indicators of the redox status in living cells, FEBS Letters, 447(2-3), pp. 135-138. https://doi.org/10.1016/S0014-5793(99)00296-3

APA Citation style: Keese, M., Saffrich, R., Dandekar, T., Becker, K., & Schirmer, R. (1999). Microinjected glutathione reductase crystals as indicators of the redox status in living cells. FEBS Letters. 447(2-3), 135-138. https://doi.org/10.1016/S0014-5793(99)00296-3