Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-κB activation - Research portal of Justus Liebig University Giessen:

Journal article

Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-κB activation

Authors list: Röder-Stolinski, C; Fischäder, G; Oostingh, GJ; Feltens, R; Kohse, F; von Bergen, M; Mörbt, N; Eder, K; Duschl, A; Lehmann, I

Publication year: 2008

Pages: 241-247

Journal: Toxicology and Applied Pharmacology

Volume number: 231

Issue number: 2

ISSN: 0041-008X

DOI Link: https://doi.org/10.1016/j.taap.2008.04.010

Publisher: Elsevier

Abstract:
Styrene is a volatile organic compound (VOC) that is widely used as a solvent in many industrial settings. Chronic exposure to styrene can result in irritation of the mucosa of the upper respiratory tract. Contact of styrene with epithelial cells stimulates the expression of a variety of inflammatory mediators, including the chemotactic cytokine monocyte chemoattractant protein-1 (MCP-1). To characterise the underlying mechanisms of the induction of inflammatory signals by styrene, we investigated the influence of this compound on the induction of oxidative stress and the activation of the nuclear factor-kappa B (NF-kappaB) signalling pathway in human lung epithelial cells (A549). The results demonstrate that styrene-induced MCP-1 expression, as well as the expression of the oxidative stress marker glutathione S-transferase (GST), is associated with a concentration dependent pattern of NF-kappaB activity. An inhibitor of NF-kappaB, IKK-NBD, and the anti-inflammatory antioxidant N-acetylcysteine (NAC) were both effective in suppressing styrene-induced MCP-1 secretion. In addition, NAC was capable of inhibiting the upregulation of GST expression. Our findings suggest that the activation of the NF-kappaB signalling pathway by styrene is mediated via a redox-sensitive mechanism.

Authors/Editors

- Uni.-Prof. Dr. Klaus Josef Eder

Citation Styles

Harvard Citation style: Röder-Stolinski, C., Fischäder, G., Oostingh, G., Feltens, R., Kohse, F., von Bergen, M., et al. (2008) Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-κB activation, Toxicology and Applied Pharmacology, 231(2), pp. 241-247. https://doi.org/10.1016/j.taap.2008.04.010

APA Citation style: Röder-Stolinski, C., Fischäder, G., Oostingh, G., Feltens, R., Kohse, F., von Bergen, M., Mörbt, N., Eder, K., Duschl, A., & Lehmann, I. (2008). Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-κB activation. Toxicology and Applied Pharmacology. 231(2), 241-247. https://doi.org/10.1016/j.taap.2008.04.010