Journalartikel

Jahr der Veröffentlichung: 1998

Seiten: 185-189

Zeitschrift: Trace Elements and Electrolytes

Bandnummer: 15

Heftnummer: 4

ISSN: 0946-2104

Verlag: Dustri-Verlag

Abstract: 
Previous studies have demonstrated that zinc deficiency changes the ratio between arachidonic acid and eicosapentaenoic acid in rat tissue phospholipids. An altered activity of phospholipase A(2) (PL A(2)), the key enzyme in membrane degradation, could be one possible reason for this phenomenon. Therefore, the present study was performed to determine the activity of this enzyme in zinc-deficient rats. Four groups of rats were fed zinc-deficient (0.5 mg Zn/kg) or zinc-adequate (45 mg Zn/kg) diets with either olive oil or linseed oil as source of fat. To ensure an adequate food intake, all the rats were force-fed by gastric tube over a period of 13 days. Feeding the zinc-deficient diets markedly lowered body weight gains as well as zinc concentrations and activities of alkaline phosphatase in plasma proving the zinc-deficient state of those animals. The activity of PL A(2) was measured in hepatic mitochondria with 1-palmitoyl-2-( 1-C-14)-linoleoyl-phosphatidylethanolamine 1-palmitoyl-2-(1-C-14)-arachidonoyl-phosphatidylethanola and 1-palmitoyl-2-(1-C-14)-arackidonoyl-phosphatidylcholine as substrates for enzyme assay. Zinc deficiency did not influence PL A(2) activity in hepatic mitochondria, regardless of the dietary fat and the substrate used. In contrast, the type of fat had a slight effect on the activity of this enzyme. Rats fed on olive oil diets had a slightly higher PL A(2) activity than rats fed on linseed oil diet with phosphatidylethanolamine as substrate. Using phosphatidylcholine as substrate, the activity of PL A(2), was generally very low. The study, in conclusion, suggests that alterations of membrane fatty acid composition observed in zinc-deficient animals are not caused by an altered activity of PL A(2).

Harvard-Zitierstil: Eder, K., Waldhauser, K. and Kirchgeßner, M. (1998) The activity of hepatic phospholipase A(2) in zinc-deficient rats, Trace Elements and Electrolytes, 15(4), pp. 185-189

APA-Zitierstil: Eder, K., Waldhauser, K., & Kirchgeßner, M. (1998). The activity of hepatic phospholipase A(2) in zinc-deficient rats. Trace Elements and Electrolytes. 15(4), 185-189.