Journal article

Publication year: 2009

Pages: 2158-2162

Journal: Journal of Chromatography B

Volume number: 877

Issue number: 22

ISSN: 1570-0232

DOI Link: https://doi.org/10.1016/j.jchromb.2009.05.048

Publisher: Elsevier

Abstract: 
For the investigation of the metabolism and biosynthesis of carnitine, sensitive determination of carnitine and its metabolic precursors, trimethyllysine and gamma-butyrobetaine, is required. We present here a new simplified method for the analysis of carnitine, its acetyl- and propyl esters, as well as trimethyllysine and gamma-butyrobetaine without need for derivatization reactions by means of normal-phase LC and electrospray ionization tandem mass spectrometry. The limits of quantification were between 5 nM for acetyl carnitine and 70 nM for carnitine. Relative standard deviations in a fivefold determination of standard solutions were between <2% for carnitine and <10% for trimethyllysine. Quantifying the formation of deuterated carnitine from deuterated gamma-butyrobetaine, this method is also suitable for the determination of the activity of gamma-butyrobetaine dioxygenase in tissues.

Harvard Citation style: Hirche, F., Fischer, M., Keller, J. and Eder, K. (2009) Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and gamma-butyrobetaine by quasi-solid phase extraction and MS/MS detection, Journal of Chromatography B, 877(22), pp. 2158-2162. https://doi.org/10.1016/j.jchromb.2009.05.048

APA Citation style: Hirche, F., Fischer, M., Keller, J., & Eder, K. (2009). Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and gamma-butyrobetaine by quasi-solid phase extraction and MS/MS detection. Journal of Chromatography B. 877(22), 2158-2162. https://doi.org/10.1016/j.jchromb.2009.05.048