Journal article

Publication year: 1990

Pages: 33-38

Journal: Free Radical Research

Volume number: 9

Issue number: 1

ISSN: 8755-0199

DOI Link: https://doi.org/10.3109/10715769009148570

Publisher: Taylor and Francis Group

Abstract: 

Among other macrophage secretory products, H₂O₂ plays an important role in the host's defense against malaria (Wozencrafl et al., Infect. Immun., 43, 664, (1984)). In our in vitro studies on the human malaria parasite Plasmodium falciparum, hydrogen peroxide was produced by the alcohol oxidase-catalyzed reaction ethanol + O₂ → acetaldehydc + H₂O₂ (EC 1.1.3.13). At concentrations of 8.7 mM (=0.5%) ethanol and 0.1 U alcohol oxidase per ml culture, more than 95% of the parasites were irreversibly damaged. Acetaldehyde was found to be parasiticidal per se - probably by releasing immature forms of P. falciparum from erythrocytes - but CH₃CHO concentrations as high as 90mM were required for complete elimination of the parasites. Ethanol (< 20mM) or alcohol oxidase alone had no significant effect on parasite viability.
As discussed, the elhanol/alcohol oxidase system might be of interest as a potential chemotherapeutic principle, especially since metabolism and pharmacology of the substrates and products are well understood.

Harvard Citation style: Becker, K., Hopkins, T. and Schirmer, R. (1990) Antimalarial activity of the ethanol/alcohol oxidase system in vitro, Free Radical Research, 9(1), pp. 33-38. https://doi.org/10.3109/10715769009148570

APA Citation style: Becker, K., Hopkins, T., & Schirmer, R. (1990). Antimalarial activity of the ethanol/alcohol oxidase system in vitro. Free Radical Research. 9(1), 33-38. https://doi.org/10.3109/10715769009148570