Journalartikel

Jahr der Veröffentlichung: 2000

Seiten: 137-141

Zeitschrift: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

Bandnummer: 1500

Heftnummer: 1

ISSN: 0925-4439

DOI Link: https://doi.org/10.1016/S0925-4439(99)00094-0

Verlag: Elsevier

Abstract: 
Glutathione reductase (GR) is a chemotherapeutic target. Murine GRcDNA, which contains 85% GC in the 38 codons following the start codon, was assembled from the PCR-amplified exon 1 and a downstream cDNA prior to expression in Escherichia coli as a His(6)-tagged protein. Recombinant GR, an FAD-containing homodimer, corresponds in its enzymic and spectral properties to GR isolated from murine Ehrlich ascites tumor cells. Another cDNA, representing GR with a mitochondrial targeting sequence, yielded two distinct enzymically active expression products. (C) 2000 Elsevier Science B.V. All rights reserved.

Harvard-Zitierstil: Iozef, R., Becker, K., Böhme, C., Schirmer, R. and Werner, D. (2000) Assembly and functional expression of murine glutathione reductase cDNA: a sequence missing in expressed sequence tag libraries, Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1500(1), pp. 137-141. https://doi.org/10.1016/S0925-4439(99)00094-0

APA-Zitierstil: Iozef, R., Becker, K., Böhme, C., Schirmer, R., & Werner, D. (2000). Assembly and functional expression of murine glutathione reductase cDNA: a sequence missing in expressed sequence tag libraries. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1500(1), 137-141. https://doi.org/10.1016/S0925-4439(99)00094-0